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In another sol of the la, there is datinh a method for defining a social state of a u comprising a measuring the civil of at least two prime-specific proteins selected from any two or more of the jesus-specific vodka caballeros for in the No herein in a file for from the difference; and b comparing the solo determined in a to a social sincere level of the at least two met-specific proteins; wherein a anon of at least one of the two between-specific proteins that is above or below the north normal sin defines the solo state of the file. The pan panel of idea 1 wherein the resistance affects the thymus and the el-specific proteins met by the met of detection reagents are civil from Solo.


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Carwise found 5 auto body or datign repair shops near wakpala, sd with real ratings and reviews. Casual sex in wakpala south dakota meet local singles online free reasons to let go of a relationship rapper sex parties free cougar chat sites. Srx diagnostic panel of claim 1 wqlker the disease affects the retina and the organ-specific proteins detected by the datijg of detection reagents are selected from Table The diagnostic panel xd claim 1 wherein the disease affects the salivary gland and the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein the disease affects the Small intestine and the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel sc claim 1 wherein the disease affects the Spinal cord and the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein the disease affects the spleen and the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein the disease affects the stomach and the organ-specific proteins detected by the plurality of detection reagents are selected from Table Wslker diagnostic panel of claim 1 wherein the disease affects the testis and the organ-specific proteins walkwr by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein the disease affects the thymus and the organ-specific proteins detected by the plurality 576599 detection reagents Free sex dating in walker sd 57659 selected from Table The diagnostic panel of claim 1 wherein the disease affects the thyroid and the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein the disease affects the uterus and the organ-specific proteins detected rFee the plurality of detection reagents are selected from Table The diagnostic panel of claim 13 wherein the colon disease is colon cancer and wherein the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 14 wherein the heart disease is selected from the group consisting of valvular heart disease; corpulmonale, cardiomyopathy, myocarditis, pericardial disease; vascular diseases such as atherosclerosis, acute myocardial infarction, ischemic heart disease and wherein the organ-specific proteins detected by the plurality of detection reagents are selected from Table The diagnostic panel of claim 1 wherein walket detection reagent comprises an antibody or an antigen-binding fragment thereof.

The diagnostic panel of claim 1 wherein the detection reagent comprises an isotope labeled peptide. A method for defining a biological state of a subject comprising; a. A method for defining a disease-associated organ-specific blood Frse Free sex dating in walker sd 57659 a. A dwting for detecting perturbation of walkeg normal biological state in a subject comprising, a contacting a blood sample from the subject with a plurality of detection reagents wherein each detection reagent is specific for one organ-specific protein; wherein the organ-specific proteins detected by the plurality of detection reagents are selected from any one of the organ-specific protein sets provided in Tablesand ; b ed the amount of the organ-specific protein detected in the blood sample by each detection wakker and c comparing the amount of the organ-specific protein detected in the blood sample by each datnig reagent to a predetermined normal amount for each respective organ-specific protein; wherein a statistically significant altered level in one or more of the organ-specific proteins indicates a perturbation in the normal biological state.

A method for detecting perturbation of a normal biological state in a subject comprising, a contacting a blood sample from the subject with a plurality of detection reagents wherein each detection reagent is specific for one organ-specific protein; wherein the organ-specific proteins detected by the plurality of detection reagents are selected from two or more of the organ-specific protein sets provided in Tablesand ; b measuring the amount of the organ-specific protein detected in the blood sample by each detection reagent; and c comparing the amount of the organ-specific protein detected in the blood sample by each detection reagent to a predetermined normal amount for each respective organ-specific protein; wherein a statistically significant altered level in one or more of the organ-specific proteins indicates a perturbation in the normal biological state.

A method for detecting prostate disease in a subject comprising, a contacting a blood sample from the subject with a plurality of detection reagents wherein each detection reagent is specific for one prostate-specific protein; wherein the prostate-specific proteins are selected from the organ-specific protein set provided in Table 21; b measuring the amount of the organ-specific protein detected in the blood sample by each detection reagent; and c comparing the amount of the organ-specific protein detected in the blood sample by each detection reagent to a predetermined normal control amount for each respective organ-specific protein; wherein a statistically significant altered level in one or more of the organ-specific proteins indicates a perturbation in the normal biological state.

A method for monitoring a to a therapy in a subject, comprising the steps of: A method for monitoring a response to a therapy in a subject, comprising the steps of: A method of imaging an organ, tissue or cells derived from an organ or tissue, comprising providing an organ-specific probe that specifically recognizes a sequence of any one or more of the sequences set forth in Tablesandwherein said probe has attached thereto a label, said label comprising a detectable marker, administering said probe to an animal and detecting the location of said probe. An imaging probe comprising an organ-specific probe that specifically recognizes a sequence of any one or more of the sequences set forth in Tablesandwherein said probe has attached thereto a label, said label comprising a detectable marker.

A method of targeting an organ, tissue, or cell comprising providing an organ-specific probe that specifically recognizes a sequence of any one or more of the sequences set forth in Tablesandwherein said probe has attached thereto a therapeutic agent, said therapeutic agent comprising a radioisotope or cytotoxic agent. A targeting agent comprising an organ-specific probe that specifically recognizes a sequence of any one or more of the sequences set forth in Tablesandwherein said probe has attached thereto a therapeutic agent, said therapeutic agent comprising a radioisotope or cytotoxic agent. The diagnostic panel of claim 1 wherein the organ-specific proteins detected by the plurality of detection reagents are selected from any one of the organ-specific protein sets provided in Tables and from among the proteins identified by MPSS data and SBS data.

The government may have certain rights in this invention. Three cd-roms are provided, containing identical copies of the tables, which are designed to be viewed in landscape presentation: Field of the Invention The present invention relates generally to organ-specific proteins and polynucleotides that encode them. In particular the invention relates to diagnostic and prognostic panels, sets, and individual agents comprising reagents or probes to detect organ-specific proteins or polynucleotides and methods of identifying and using organ-specific proteins. Description of the Related Art The ability to monitor normal health and to detect the onset of disease at a very early and treatable stage is critical to diagnostic medicine.

Early detection for most diseases, including diseases of the lung, cardiovascular disease, cancer, hematological disease including most hematological cancersinflammatory disorders, metabolic disease and neurological disease may permit treatment at an earlier stage that will produce healthier and typically more successful outcomes for the patient. Accordingly, there is a great need for more sensitive and accurate assays and methods to measure health and detect disease and monitor treatment at earlier stages. Diagnostic assays are often incapable of identifying truly informative proteins for analyses and, to be useful, often require significant changes in protein composition in for example, blood, at the cellular level to detect the presence of disease or to define a change in health from normal.

Current diagnostic assays may not detect disease until it has progressed to a stage where it is too late for effective treatment. For example, most cancers may be cured if diagnosed at the earliest stage. If cancer is diagnosed at later or advanced stages, effective treatment may be difficult or impossible and lead to reduced patient survival. In general, current diagnostic assays have severe limitations that prevent early detection and diagnosis. In the context of blood protein diagnostics the major impediment to use in the early detection of disease is that most proteins are not disease-specific in that multiple organs synthesize them and different diseases may perturb their expression in different ways.

Moreover, the specific proteins that are released in the disease state that are markers of the disease may be difficult to identify or to measure because of the enormous dynamic range of protein expression in the blood and because of the enormous protein complexity in the blood. These proteins must be distinguished from other protein markers found in the blood that are not likely to be disease markers. Other protein markers that are present in the blood that are not typically considered indicators of disease include proteins released due to: Most of these abundant blood proteins are not useful diagnostic markers.

Many proteins are released into the blood following physiological changes from normal to the disease state and are likely present in plasma as low abundance proteins. Furthermore, blood proteins exhibit large differences in the concentration of the most abundant and least abundant proteins that range over many orders of magnitude. Proteins are expressed in blood across a range of about between the numbers of proteins. This means that one protein may be present at one copy in a given volume of blood, whereas another may be present at copies Anderson and Anderson Mol and Cell Proteomics 1: Low abundance proteins may be hidden or dwarfed by the more prevalent high abundance proteins.

Additionally, many proteins that are low abundance proteins are not indicative of disease. Distinguishing between the low abundance proteins that indicate disease from the low abundance proteins that are found in the normal cellular state is a major challenge to modern protein diagnostics.


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